Royal HFX probe - a new tool for drug composition analysis developed by Japan Electronics

Jeol USA has developed a new tool, the Royal HFX probe, which has unique value in the analysis of complex drug mixtures containing various metabolites. It is a familiar problem for pharmacists to separate drugs containing mixed substances.

At present, many synthetic drug molecules contain fluorine, but this element is rare in organic system, so all fluorine-free molecules can be filtered by 19F NMR, which provides an effective means for the specific screening of complex mixtures.

Last year, universities in the UK and Brazil, together with a large pharmaceutical company, published a study proposing a method called sri-festa. After the polarization transfer from 19F to 1H, a single 1H was selectively labeled. The experiment ended with TOCSY transfer to reveal all the protons coupled with the selected 1H.

Sri-festa has powerful function and strong selectivity, but it needs professional operation when selecting specific excitation points of 19F and 1H, which may limit its practical application in conventional drug laboratories.

Now, on the basis of this experimental method, using the unique characteristics of Royal HFX probe, jeol scientists have come up with a completely different method to replace the need for selective pulse - the use of broadband pulse. Although a complex initial spectrum can be obtained by this method, the desired path can be selected by modifying the phase period to obtain ideal and clean results.

As far as we know, this is an unprecedented new way to use isotope filtration," said jeol USA. Scientists can do a simple and clear experiment, which can effectively reveal the 1H TOCSY filtered by 19F in the mixture, without requiring the NMR operator to first identify and then select the location of band selection. I believe that our new method has great potential in drug development, and we are glad to see that this technology can be used to simplify the experimental operation.

Royal HFX probe is the first one in the world that can operate 1H, 19F and 13C spin liquid NMR probes at the same time. Compared with the traditional NMR probe designed by proton fluorine atomic NMR, it has no typical loss in performance. It provides the flexibility to simplify the modern complex spectral distribution of fluorine-containing compounds, and magnetic tuning enables the probe to achieve the highest performance of 1H or 19F in single tuning mode.