Synthetic Colonic Mucus Enables the Development of Modular Microbiome Organoids

The Title of this article is "Synthetic Colonic Mucus Enables the Development of Modular Microbiome Organoids". The main research contents and findings include:
1.Research Background:
It introduced the complexity of the human colonic microbiome and its roles in nutrient metabolism, immune regulation, and disease prevention. Meanwhile, it was pointed out that the research on how these microorganisms affect human physiology has been limited by experimental tools.
2.Synthetic Mucus Material (FAMS):
The researchers developed a synthetic gel-like material that can mimic the structural, mechanical, and biochemical properties of human colonic mucus. This material is achieved by the self-assembly of a fluorine-rich amino acid at the water-liquid phase interface.
3.Properties of FAMS: 
Structure: FAMS has a bilayer structure similar to that of colonic mucus, including a dense inner layer that adheres to epithelial cells and a diffuse outer layer that contains symbiotic bacteria.
Mechanical Properties: The rheological properties of FAMS can be adjusted by changing the ionic strength and pH value of the environment to simulate the mechanical behavior of natural mucus.
Chemical Properties: FAMS can bind to mucin to form an environment suitable for microbial colonization and growth.
4.Microbiome Organoids:
The researchers combined FAMS with human colonic cells to generate an artificial mucosa, called microbiome organoids. These organoids can integrate the microbiome to form a multicellular system that simulates the natural colonic environment.
5.Application Potential:
Drug Screening: The FAMS platform can be used in high-throughput screening methods to study compound metabolism and intestinal permeability.
Microbiology Research: FAMS is helpful for promoting probiotic research and providing information for in vivo methods.
6.Experimental Methods:
It described in detail the experimental methods such as the preparation of synthetic mucus, the measurement of rheological properties, fluorescence spectroscopy and imaging, bacterial growth and FAMS inoculation, electron microscopy and confocal imaging, transmembrane diffusion and cell detachment.
7.Research Results:
It demonstrated the effectiveness of FAMS in simulating the intestinal microbiome environment and its application in drug absorption and permeability testing.



Key words:
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